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Proteomic analysis Cells were seeded in 10-cm dishes and cultured for 7 days in cystine-free medium supplemented with heavy isotope-labelled 13 C 6 , 15 N 2 -cystine (Cambridge Isotope Laboratories, CNLM-4244-H)

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Considering a three-dimensional structural model for a complex of the human HGF-NK2 segment sandwiched by c-met receptor molecules (see supplementary Figure 3, re-produced from PDB ID 7MO7 67 , it is hypothesized that the disulfide bonds, notably Cys149-Cys189 and Cys177-Cys201 localized internally just behind Y198, are the targets of LASSS